atcc 44175  (ATCC)

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: Amplification of ITS region from DNA isolated from pure fungal cultures

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Staining

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: PCR amplification of nuclear rDNA from total DNA isolated from pure cultures of basidiomycetes (A) and ascomycetes (B). Electrophoresis in 2% (wt/vol) agarose in 1× TBE. The two outer lanes contain molecular weight markers. Inner even-numbered lanes contain samples amplified by the primer pair ITS1-F and ITS4-B, and the odd-numbered lanes contain samples amplified by primers ITS1-F and ITS4. (A) Lanes 2 to 9 contain brown-rot fungi, and lanes 10 to 17 contain white-rot fungi. Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Coniophora puteana Fp-90099-Sp; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Postia placenta Mad-698-R; lanes 8 and 9, Serpula lacrimans Harm-888-R; lanes 10 and 11, Lentinula edodes 117=1t(d); lanes 12 and 13, Resinicium bicolor ATCC 64897; lanes 14 and 15, Scytinostroma galactinum ATCC 64896; lanes 16 and 17, Trametes versicolor Fp-101664-Sp; lanes 18 and 19, no template DNA (i.e., negative controls). (B) Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Aureobasidium pullulans ATCC 34621; lanes 4 and 5, Hormonema dematiodes; lanes 6 and 7, Pestalotiopsis sp.; lanes 8 and 9, Leucostoma kunzei; lanes 10 and 11, Scleroderris lagerbergii 1877; lanes 12 and 13, Sirococcus conigenus; lanes 14 and 15, Sphaeropsis sapinea B468; lanes 16 and 17, Xenomeris abietis; lanes 18 and 19, no template DNA (i.e., negative controls).

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Electrophoresis, Molecular Weight

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: Wood decay and detection of fungal species in wood blocks after 8 months of colonization

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: TaqI restriction digests of the PCR product amplified by the primer pair ITS1-F and ITS4-B from DNA isolated from pure cultures of basidiomycetes. Electrophoresis in 2% (wt/vol) Sepharide Gel Matrix (Gibco-BRL) in 1× TAE. The two outer lanes contain molecular weight markers. Each inner lane contains a different fungal species; lanes 2 to 8 contain brown-rot fungi, and lanes 9 to 15 contain white-rot fungi. Lanes 1 and 18, PCR markers (Promega); lane 2, Coniophora puteana Fp-90099-Sp; lane 3, Fomitopsis pinicola K8sp; lane 4, Gloeophyllum sepiarum 10-BS2-2; lane 5, Gloeophyllum trabeum Mad-617-R; lane 6, Leucogyrophana pinastri; lane 7, Postia placenta Mad-698-R; lane 8, Serpula lacrimans Harm-888-R; lane 9, Irpex lacteus KTS 003; lane 10, Lentinula edodes 117=1t(d); lane 11, Phanerochaete chrysosporium ATCC 24725; lane 12, Resinicium bicolor ATCC 64897; lane 13, Scytinostroma galactinum ATCC 64896; lane 14, Trametes versicolor Fp-101664-Sp; lane 15, Trichaptum abietinum 1247 MJL; lane 16, Pisolithus tinctorium ATCC 38054, an ectomycorrhiza; lane 17, Rhizoctonia solani 1AP, a pathogen of herbaceous plants.

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Electrophoresis, Molecular Weight

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: TaqI restriction digests of the PCR product amplified by the primer pair ITS1-F and ITS4-B from DNA isolated from wood decay basidiomycetes. Electrophoresis in 2% (wt/vol) Sepharide Gel Matrix (Gibco-BRL) in 1× TAE. The two outer lanes contain molecular weight markers. Each group of three inner lanes represents the TaqI digests for one fungal isolate amplified from DNA isolated from each of two different wood blocks and a pure culture from left to right, respectively. (A) Lanes 1 and 20, PCR markers (Promega); lanes 2 to 4, Gloeophyllum trabeum; lanes 5 to 7, Gloeophyllum trabeum, Mad-617-R; lanes 8 to 10, Postia placenta; lanes 11 to 13, Postia placenta Mad-698-R; lanes 14 to 16, Trametes versicolor; lanes 17 to 19, Trametes versicolor Fp-101664-Sp. (B) Lanes 1 and 20, PCR markers (Promega); lanes 2 to 4, Resinicium bicolor; lanes 5 to 7, Resinicium bicolor ATCC 44175; lanes 8 to 10, Resinicium bicolor ATCC 64897; lanes 11 to 13, Scytinostroma galactinum; lanes 14 to 16, Scytinostroma galactinum ATCC 64896; lanes 17 to 19, Scytinostroma galactinum ATCC 44178.

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Electrophoresis, Molecular Weight

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: Amplification of ITS region from DNA isolated from pure fungal cultures

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Staining

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: PCR amplification of nuclear rDNA from total DNA isolated from pure cultures of basidiomycetes (A) and ascomycetes (B). Electrophoresis in 2% (wt/vol) agarose in 1× TBE. The two outer lanes contain molecular weight markers. Inner even-numbered lanes contain samples amplified by the primer pair ITS1-F and ITS4-B, and the odd-numbered lanes contain samples amplified by primers ITS1-F and ITS4. (A) Lanes 2 to 9 contain brown-rot fungi, and lanes 10 to 17 contain white-rot fungi. Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Coniophora puteana Fp-90099-Sp; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Postia placenta Mad-698-R; lanes 8 and 9, Serpula lacrimans Harm-888-R; lanes 10 and 11, Lentinula edodes 117=1t(d); lanes 12 and 13, Resinicium bicolor ATCC 64897; lanes 14 and 15, Scytinostroma galactinum ATCC 64896; lanes 16 and 17, Trametes versicolor Fp-101664-Sp; lanes 18 and 19, no template DNA (i.e., negative controls). (B) Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Aureobasidium pullulans ATCC 34621; lanes 4 and 5, Hormonema dematiodes; lanes 6 and 7, Pestalotiopsis sp.; lanes 8 and 9, Leucostoma kunzei; lanes 10 and 11, Scleroderris lagerbergii 1877; lanes 12 and 13, Sirococcus conigenus; lanes 14 and 15, Sphaeropsis sapinea B468; lanes 16 and 17, Xenomeris abietis; lanes 18 and 19, no template DNA (i.e., negative controls).

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Electrophoresis, Molecular Weight

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: Wood decay and detection of fungal species in wood blocks after 8 months of colonization

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Control

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: TaqI restriction digests of the PCR product amplified by the primer pair ITS1-F and ITS4-B from DNA isolated from pure cultures of basidiomycetes. Electrophoresis in 2% (wt/vol) Sepharide Gel Matrix (Gibco-BRL) in 1× TAE. The two outer lanes contain molecular weight markers. Each inner lane contains a different fungal species; lanes 2 to 8 contain brown-rot fungi, and lanes 9 to 15 contain white-rot fungi. Lanes 1 and 18, PCR markers (Promega); lane 2, Coniophora puteana Fp-90099-Sp; lane 3, Fomitopsis pinicola K8sp; lane 4, Gloeophyllum sepiarum 10-BS2-2; lane 5, Gloeophyllum trabeum Mad-617-R; lane 6, Leucogyrophana pinastri; lane 7, Postia placenta Mad-698-R; lane 8, Serpula lacrimans Harm-888-R; lane 9, Irpex lacteus KTS 003; lane 10, Lentinula edodes 117=1t(d); lane 11, Phanerochaete chrysosporium ATCC 24725; lane 12, Resinicium bicolor ATCC 64897; lane 13, Scytinostroma galactinum ATCC 64896; lane 14, Trametes versicolor Fp-101664-Sp; lane 15, Trichaptum abietinum 1247 MJL; lane 16, Pisolithus tinctorium ATCC 38054, an ectomycorrhiza; lane 17, Rhizoctonia solani 1AP, a pathogen of herbaceous plants.

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Electrophoresis, Molecular Weight

Journal:

Article Title: Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA †

doi:

Figure Lengend Snippet: TaqI restriction digests of the PCR product amplified by the primer pair ITS1-F and ITS4-B from DNA isolated from wood decay basidiomycetes. Electrophoresis in 2% (wt/vol) Sepharide Gel Matrix (Gibco-BRL) in 1× TAE. The two outer lanes contain molecular weight markers. Each group of three inner lanes represents the TaqI digests for one fungal isolate amplified from DNA isolated from each of two different wood blocks and a pure culture from left to right, respectively. (A) Lanes 1 and 20, PCR markers (Promega); lanes 2 to 4, Gloeophyllum trabeum; lanes 5 to 7, Gloeophyllum trabeum, Mad-617-R; lanes 8 to 10, Postia placenta; lanes 11 to 13, Postia placenta Mad-698-R; lanes 14 to 16, Trametes versicolor; lanes 17 to 19, Trametes versicolor Fp-101664-Sp. (B) Lanes 1 and 20, PCR markers (Promega); lanes 2 to 4, Resinicium bicolor; lanes 5 to 7, Resinicium bicolor ATCC 44175; lanes 8 to 10, Resinicium bicolor ATCC 64897; lanes 11 to 13, Scytinostroma galactinum; lanes 14 to 16, Scytinostroma galactinum ATCC 64896; lanes 17 to 19, Scytinostroma galactinum ATCC 44178.

Article Snippet: Lanes 1 and 20, PCR markers (Promega); lanes 2 and 3, Postia placenta Mad-698-R; lanes 4 and 5, Gloeophyllum trabeum Mad-617-R; lanes 6 and 7, Leucogyrophana pinastri ; lanes 8 and 9, Lentinula edodes 117=1t(d); lanes 10 and 11, Trametes versicolor ; lanes 12 and 13, Scytinostroma galactinum ATCC 64896; lanes 14 and 15, Hormonema dematiodes ; lanes 16 and 17, Pestalotiopsis sp. ; lanes 18 and 19, no template DNA (i.e., negative controls). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Isolate Mean % wt loss of wood ± SD a PCR amplification b with primer pair: ITS1-F–ITS4 ITS1-F–ITS4-B Uninoculated control wood 0.1 ± 0.3 ++ −− Brown-rot basidiomycetes Coniophora puteana Fp-90099-Sp 0.1 ± 0.3 ++ ++ Postia placenta Mad-698-R 65.5 ± 1.2 ++ ++ Postia placenta 65.8 ± 1.4 ++ ++ Gloeophyllum trabeum 67.3 ± 3.3 ++ ++ Gloeophyllum trabeum Mad-617-R 69.6 ± 1.8 ++ ++ Gloeophyllum sepiarum 10-BS2-2 68.1 ± 0.9 ++ ++ Leucogyrophana pinastri 68.1 ± 4.8 ++ ++ Serpula lacrimans Harm-888-R 67.5 ± 1.9 ++ ++ White-rot basidiomycetes Lentinula edodes 117=1t(d) 3.0 ± 1.7 ++ ++ Trametes versicolor 35.1 ± 13.6 ++ ++ Trametes versicolor Fp-101664-Sp 0.0 ± 0.1 ++ ++ Irpex lacteus KTS 003 40.1 ± 12.1 ++ ++ Resinicium bicolor HHB-8850-sp 10.4 ± 0.9 ++ ++ Resinicium bicolor ATCC 44175 3.7 ± 3.6 ++ ++ Resinicium bicolor ATCC 64897 12.1 ± 5.7 ++ ++ Scytinostroma galactinum MB-1880-sp −0.8 ± 0.5 ++ ++ Scytinostroma galactinum ATCC 64896 1.1 ± 0.3 ++ ++ Scytinostroma galactinum ATCC 44178 −0.2 ± 0.7 ++ ++ Phanerochaete chrysosporium 9.9 ± 11.8 ++ ++ Phanerochaete chrysosporium ATCC 24725 14.2 ± 5.7 ++ ++ Wood-inhabiting ascomycetes Phialophora mutabilis ATCC 42792 0.4 ± 0.2 ++ −− Trichoderma reesei ATCC 26921 −0.2 ± 0.4 ++ −− Trichoderma viride ATCC 32630 −0.1 ± 0.1 ++ −− Hormonema dematiodes 0.4 ± 0.0 ++ −− Pestolotiopsis sp. 0.6 ± 0.0 ++ −− Xenomeris abietis 0.5 ± 0.0 ++ −− Aureobasidium pullulans ATCC 34621 0.2 ± 0.0 ++ −− Phialocephala fusca ATCC 62326 −0.2 ± 0.0 ++ −− Ceratocystis pilifera ATCC 60758 2.7 ± 0.0 ++ −− Ophiostoma ulmi ATCC 32439 0.1 ± 0.0 ++ −− Open in a separate window a Mean of two replicate spruce wood blocks. b Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes.

Techniques: Amplification, Isolation, Electrophoresis, Molecular Weight